ABSTRACT
Objective:To investigate the effect of sleeve gastrectomy on blood glucose in obese rats with diabetes mellitus.Methods:Thirty-two 12 week old Goto Kakizaki (GK) diabetic rats were randomly divided into 4 groups: sham operation group (A) , sleeve stomach operation group (B) , sleeve stomach operation+external bile drainage group (C) and sleeve stomach operation + external bile drainage + oleanolic acid group (D) . The changes of fasting blood glucose and blood bile acid were compared before and after operation. The expression level of GLP-1 in serum of each group was detected by ELISA, and the difference of protein expression of bile acid G protein coupled receptor (TGR5) was detected by Western blot.Results:The blood glucose level of group A had no significant change after operation. But blood glucose level in group B and group D was significantly lower than that before operation. Blood glucose in group C was lower than that before operation, but there was no significant difference in comparison. Serum total bile acid level in group A had no significant difference before and after operation. Bile acid level in group B was significantly higher than that before operation, while bile acid level in group C and group D was significantly lower than that before operation. There was no significant difference in the level of serum GLP-1 in group A before and after operation. The level of serum GLP-1 in group B and group D was significantly higher than that before operation. The level of serum GLP-1 in group C was lower than that before operation, but there was no significant difference. The expression of TGR5 protein in terminal ileum in group B and group D was higher than that in group A, but the expression in group C was similar with that in group A.Conclusions:Sleeve gastrectomy has definite hypoglycemic effect on T2DM rats. Bile acid and its related receptor TGR5 should play an important role in the mechanism of sleeve gastrectomy in treatment of diabetes.
ABSTRACT
OBJECTIVE To investigate and analyze the risk factors,clinical characteristics and prevention countermeasures of hospital fungal incfection in old patients with malignant tumors.METHODS To adopt investigation way to review and analyze the clinical data of hospital fungal infection in 165 cases from the old patients with malignant tumors.RESULTS Among 165 cases the infection occurred predominantly in lower respiratory tract(47.2%),and with urological tract being second(19.0%).Of 165 strains of fungi from 165 patients,the first was Candida albicans(55.8%),and the second was C.tropicalis(23.0%).CONCLUSIONS The causes of hospital fungal infection in old patients with malignant tumors are highly associated with the risk factors such as primary tumor,age,radiotherapy,chemotherapy,antibiotics,hormone,invasive operation,and so on.